Description

The rabbit monoclonal antibody R19M specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

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Item Information
Catalog #	200106
Size/Concentration	100 Tests
Price	$1,750
Antibody Registration Number	AB_2857947

 

Specification
Host Species	Rabbit
Antibody Type	Monoclonal
Antigen Purification	Protein A
Reactivity	Mouse
Immunogen	scFv region of a CD19-specific mouse mAb clone FMC63.
Storage Buffer	The aqueous buffered solution contains protein stabilizer and ≤0.03% sodium azide.
Fluorescent Dye	R-PE
Excitation (nm)	565
Application	FCM

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Shipping and Storage 

Shipping	The product is shipped at 4°C.
Stability & Storage	To provide optimal stability of reagent:    Store at 4°C for 2 weeks  Protect from prolonged exposure to light.  Do not freeze.

 

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FACS Protocol 

• Reagents Required 

• • Phosphate Buffered Saline (PBS with a pH ~7.4).  
  • FACS Buffer (PBS containing 2% of BSA).  

• • Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (R19M), PE (100 tests) 

• Preparation of Reagents 

• • Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (R19M), PE (100 tests) 

• • • Protein must be diluted before use for FACS. 
    • Dilute the protein at a dilution of 1:100 in FACS buffer. 

• Staining Protocol 

1. Harvest the cells from source and wash the cells once using FACS buffer. 
2. Perform a cell count and a cell viability count. Cell viability must be ≥ 95% with a cell number above 2×105 live cells. 
3. In a round-bottom test tube, resuspend cells in 100 µL of diluted Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (R19M), PE for 30 min at 4°C. 
4. Wash the mixture of cells and antibody 3 times by centrifuge using FACS buffer. 
5. Resuspend the stained cells in 200 µL PBS per cell sample.  
6. Transfer the stained cell into a desired flow tube for analyzation on desired Flow Cytometer. The recommended acquisition rate is >10,000 events. 

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Product Notices

• Since applications vary, each investigator should titrate the reagent to obtain optimal results.
• Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

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Intellectual Product Notices

For Research Use Only. Not for use in diagnostic or therapeutic procedures

1. This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer and the buyer must not use this product or its components:
   for:
   1. diagnostic, therapeutic or prophylactic purposes; or
   2. manufacturing or quality assurance or quality control.
   3. and/or to sell or transfer this product or its components for resale, whether or not resold for use in research.
2. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
3. The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents. Bioswan will not be held responsible for patent infringement or other violations that may occur with the use of our products.
4. Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product.
5. Any use of this product other than the permitted use without the express written authorization of BioSwan Company is strictly prohibited.
   Not for resales. BioSwan, the BioSwan Logo and all other trademarks are property of BioSwan Laboratories, Co., Ltd..