Description

Product Description

BCMA (B-cell maturation antigen)/ TNFRSF17 (tumor necrosis factor receptor superfamily member 17) is a member of the TNF-receptor superfamily and are expressed preferentially expressed in mature B lymphocytes and may be important for B cell development and autoimmune response. They are also expressed on the surface of cancer cells. The Human BCMA/TNFRSF17 Protein is specifically designed to mimic these receptors, allowing it to be used in CAR T-cell therapy research

The CAR consists of an extracellular domain that recognizes the BCMA/TNFRSF17 protein, a transmembrane domain, and an intracellular domain that activates the T-cell when engaged by the target antigen. The Human BCMA/TNFRSF17 Protein is employed to evaluate the effectiveness of CAR T-cells in targeting and killing cancer cells expressing BCMA/TNFRSF17.

The addition of FC enhances the stability and solubility of the protein while incorporating Alexa Fluor 647, allows for a photostable antibody for precise visualization and detection of the protein in CAR T-cell assays.

 

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Item Information
Catalog #	401502
Size/Concentration	100 Test
Price	$680.00

 

Specification
Gene Name Synonym	BCMA; CD269; TNFRSF17
Source	Human
Expression Host	ExpiCHO cells
Antigen Purification	Protein A
Fluorescent Dye	Alexa Fluor 647
Excitation (nm)	650
Molecular Mass	The recombinant human TNFRSF17 consists of 303 amino acids and predicts a molecular mass of 33.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human TNFRSF17 is approximately 33-44 kDa due to the glycosylation.
Storage Buffer	Aqueous buffered solution containing protein stabilizer and ≤0.05% ProClin 300.
Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.

 

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Preparation and Storage

Shipping	The product is shipped at 2-8°C.
Stability & Storage	To provide optimal stability of reagent:   Store undiluted at 2-8°C  Avoid prolonged exposure to light  Avoid repeated freeze-thaw cycles.

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FACS Protocol

• Reagents Required
  • Phosphate Buffered Saline (PBS with a pH ~7.4). 
  • FACS Buffer (PBS containing 2% of BSA). 
  • Human BCMA / TNFRSF17 Protein, Fc Tag, Alexa Fluor 647
• Preparation of Reagents
  1. Human BCMA / TNFRSF17 Protein, Fc Tag, Alexa Fluor 647
     • Protein must be diluted before use for FACS.
     • Dilute the protein at a dilution of 1:100 in FACS buffer. 
• Staining Protocol
  1. Harvest the cells from source and wash the cells once using FACS buffer. 
  2. Perform a cell count and a cell viability count. Cell viability must be ≥ 95% with a cell number above 2×105 live cells.
  3. In a round-bottom test tube, resuspend cells in 100 µL of diluted Human BCMA / TNFRSF17 Protein, Fc Tag, Alexa Fluor 647 for 30 min at 4°C.
  4. Wash the mixture of cells and antibody 3 times by centrifuge using FACS buffer.
  5. Resuspend the stained cells in 200 µL PBS per cell sample.  
  6. Transfer the stained cell into a desired flow tube for analyzation on desired Flow Cytometer. The recommended acquisition rate is >10,000 events. 

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Product Notices

1. Since applications vary, optimal titration should be determined by each investigator for each application to obtain optimal results.
2. Caution: Antibody solutions containing ProClin 300 should be handled with care. Do not take internally and avoid all contact with the skin, mucosa
   and eyes.

Intellectual Product Notices

For Research Use Only. Not for use in diagnostic or therapeutic procedures

1. This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer and the buyer must not use this product or its components:
   for:
   1. diagnostic, therapeutic or prophylactic purposes; or
   2. manufacturing or quality assurance or quality control.
   3. and/or to sell or transfer this product or its components for resale, whether or not resold for use in research.
2. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
3. The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents. Bioswan will not be held responsible for patent infringement or other violations that may occur with the use of our products.
4. Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product.
5. Any use of this product other than the permitted use without the express written authorization of BioSwan Company is strictly prohibited.
   Not for resales. BioSwan, the BioSwan Logo and all other trademarks are property of BioSwan Laboratories, Co., Ltd..