Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody, Biotin (100 Tests)

Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody, Biotin (100 Tests)


Flow cytometric analysis of anti-CD19 CAR expression on human cell line Jurkat cells. Jurkat cells were transduced with lentivirus encoding anti-CD19 CAR and cultured. 2×105 cells were stained for the expression of anti-CD19 CAR with Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody, Biotin (Cat. No. 500014, right panel). Secondary staining was carried out with Streptavidin PE (Cat. No.700032). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel). Acquisition of >10, 000 events was performed.


The rabbit polyclonal antibody R19P specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.


Item Information 

Catalog #  500014 
Size/Concentration  100 tests 
Price  $500.00
Antibody Registration Number  AB_2857948 



Host Species  Rabbit 
Antibody Type  Polyclonal
Antigen Purification  Protein A 
Reactivity  Mouse 
Immunogen  scFv region of a CD19-specific mouse mAb clone FMC63 
Storage Buffer  Aqueous buffered solution containing protein stabilizer and ≤0.05% ProClin 300
Application  FCM 


Shipping and Storage

Shipping   The product is shipped at 2-8°C.  
Stability & Storage   To provide optimal stability of reagent:   

  • Store undiluted at 4°C 
  • Avoid prolonged exposure to light 
  • Avoid freezing the reagent  

FACS Protocol 

  • Reagents Required 
    • Phosphate Buffered Saline (PBS with a pH ~7.4).  
    • FACS Buffer (PBS containing 2% of BSA).  
    • Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody, Biotin (100 Tests) 
  • Preparation of Reagents 
    • Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody, Biotin (100 Tests) 
      • Protein must be diluted before use for FACS. 
      • Dilute the protein at a dilution of 1:100 in FACS buffer. 
  • Staining Protocol 
  1. Harvest the cells from source and wash the cells once using FACS buffer. 
  2. Perform a cell count and a cell viability count. Cell viability must be ≥ 95% with a cell number above 2×105 live cells. 
  3. In a round-bottom test tube, resuspend cells in 100 µL of diluted Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody, Biotin for 30 min at 4°C. 
  4. Wash the mixture of cells and antibody 3 times by centrifuge using FACS buffer. 
  5. Resuspend the stained cells in 200 µL PBS per cell sample.  
  6. Transfer the stained cell into a desired flow tube for analyzation on desired Flow Cytometer. The recommended acquisition rate is >10,000 events. 

Product Notices

  1. Since applications vary, optimal titration should be determined by each investigator for each application to obtain optimal results.
  2. Caution: Antibody solutions containing ProClin 300 should be handled with care. Do not take internally and avoid all contact with the skin, mucosa
    and eyes.

Intellectual Product Notices

For Research Use Only. Not for use in diagnostic or therapeutic procedures

  1. This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer and the buyer must not use this product or its components:

    1. diagnostic, therapeutic or prophylactic purposes; or
    2. manufacturing or quality assurance or quality control.
    3. and/or to sell or transfer this product or its components for resale, whether or not resold for use in research.
  2. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
  3. The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents. Bioswan will not be held responsible for patent infringement or other violations that may occur with the use of our products.
  4. Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product.
  5. Any use of this product other than the permitted use without the express written authorization of BioSwan Company is strictly prohibited.
    Not for resales. BioSwan, the BioSwan Logo and all other trademarks are property of BioSwan Laboratories, Co., Ltd..


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