The rabbit monoclonal antibody R19M specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
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Item Information |
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| Catalog # | 200106 |
| Size/Concentration | 100 Tests |
| Price | $1,750 |
| Antibody Registration Number | AB_2857947 |
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Specification |
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| Host Species | Rabbit |
| Antibody Type | Monoclonal |
| Antigen Purification | Protein A |
| Reactivity | Mouse |
| Immunogen | scFv region of a CD19-specific mouse mAb clone FMC63. |
| Storage Buffer | The aqueous buffered solution contains protein stabilizer and ≤0.03% sodium azide.
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| Fluorescent Dye | R-PE |
| Excitation (nm) | 565 |
| Application | FCM |
Shipping and Storage
| Shipping | The product is shipped at 4°C. |
| Stability & Storage | To provide optimal stability of reagent:
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FACS Protocol
- Reagents Required
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- Phosphate Buffered Saline (PBS with a pH ~7.4).
- FACS Buffer (PBS containing 2% of BSA).
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- Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (R19M), PE (100 tests)
- Preparation of Reagents
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- Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (R19M), PE (100 tests)
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- Protein must be diluted before use for FACS.
- Dilute the protein at a dilution of 1:100 in FACS buffer.
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- Staining Protocol
- Harvest the cells from source and wash the cells once using FACS buffer.
- Perform a cell count and a cell viability count. Cell viability must be ≥ 95% with a cell number above 2×105 live cells.
- In a round-bottom test tube, resuspend cells in 100 µL of diluted Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (R19M), PE for 30 min at 4°C.
- Wash the mixture of cells and antibody 3 times by centrifuge using FACS buffer.
- Resuspend the stained cells in 200 µL PBS per cell sample.
- Transfer the stained cell into a desired flow tube for analyzation on desired Flow Cytometer. The recommended acquisition rate is >10,000 events.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Intellectual Product Notices
- Conditions: The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents. BioSwan will not be held responsible for patent infringement or other violations that may occur with the use of our products. Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of BioSwan Company is strictly prohibited. For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resales. BioSwan, the BioSwan Logo and all other trademarks are property of BioSwan Laboratories, Co., Ltd.
Flow Cytometry FACS Protocol
- Reagents Required
- Phosphate Buffered Saline (PBS with a pH ~7.4).
- FACS Buffer (PBS containing 2% of BSA).
- Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (R19M), PE
- Preparation of Reagents
-
- Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (R19M), PE
- The antibody must be diluted before use for FACS.
- Dilute the antibody at a dilution of 1:100 in FACS buffer.
- Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (R19M), PE
- Staining Protocol
-
- Harvest the cells from source and wash the cells once using FACS buffer.
- Perform a cell count and a cell viability count. Cell viability must be ≥ 95% with a cell number above 2×105 live cells.
- In a round-bottom test tube, resuspend cells in 100 µL of diluted Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (R19M), PE for 30 min at 4°C.
- Wash the mixture of cells and antibody 3 times by centrifuge using FACS buffer.
- Resuspend the stained cells in 200 µL PBS per cell sample.
- Transfer the stained cell into a desired flow tube for analyzation on desired Flow Cytometer. The recommended acquisition rate is >10,000 events.
Citations List
- Agarwal S, Aznar MA, Rech AJ, Good CR, Kuramitsu S, Da T, Gohil M, Chen L, Hong SA, Ravikumar P, Rennels AK, Salas-Mckee J, Kong W, Ruella M, Davis MM, Plesa G, Fraietta JA, Porter DL, Young RM, June CH. Deletion of the inhibitory co-receptor CTLA-4 enhances and invigorates chimeric antigen receptor T cells. Immunity. 2023 Oct 10;56(10):2388-2407.e9. doi: 10.1016/j.immuni.2023.09.001. Epub 2023 Sep 29. PMID: 37776850; PMCID: PMC10591801.
- Agarwal S, Wellhausen N, Levine BL, June CH. Production of Human CRISPR-Engineered CAR-T Cells. J Vis Exp. 2021 Mar 15;(169). doi: 10.3791/62299. PMID: 33779622.
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Preclinical proof of concept for VivoVec, a lentiviral-based platform for in vivo CAR T-cell engineering
- Wu G, Guo S, Luo Q, Wang X, Deng W, Ouyang G, Pu JJ, Lei W, Qian W. Preclinical evaluation of CD70-specific CAR T cells targeting acute myeloid leukemia. Front Immunol. 2023 Feb 10;14:1093750. doi: 10.3389/fimmu.2023.1093750. PMID: 36845088; PMCID: PMC9950117.
