Description
The rabbit monoclonal antibody R19M specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
| Item Information | |||
|---|---|---|---|
| Catalog # | Size/concentration | Price | Antibody Registration Number |
| 200105 | 25 tests | $480 | AB_2857947 |
| Specification | |
|---|---|
| Host species | Rabbit |
| Antibody type | Monoclonal |
| Immunogen | scFv region of a CD19-specific mouse mAb clone FMC63 |
| Reactivity | Mouse |
| Storage buffer | Aqueous buffered solution containing protein stabilizer and ≤0.03% sodium azide |
| Application | FCM |
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified by Protein A.
The antibody was conjugated with R-PE under optimum conditions.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
FACS Protocoal
- Harvest the cells and wash the cells once by FACS buffer (PBS containing 2% of BSA).
- Count the cells number and the viability, aliquot up to 2×105 live cells into each tube. (Note: the cell viability must be ≥ 95%.)
- Resuspend cells in 100 µL of diluted Anti-Mouse FMC63 scFv mAb (R19M), PE (Cat. No. 200105, 1:100 diluted in FACS buffer) for 30 min at 4°C.
- Wash the cells 3 times by FACS buffer and resuspend the cells in 200 µL PBS per sample.
- Transfer the cells into flow tube and analyze on Flow Cytometer. Acquisition of >10, 000 events is performed.
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