The rabbit monoclonal antibody R19M specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
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Item Information |
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| Catalog # | 200124 |
| Size/Concentration | 100 Tests |
| Price | $1,950 |
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Specification |
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| Host Species | Rabbit |
| Antibody Type | Monoclonal |
| Antigen Purification | Protein A |
| Reactivity | Mouse |
| Immunogen | scFv region of a CD19-specific mouse mAb clone FMC63. |
| Storage Buffer | The aqueous buffered solution contains protein stabilizer and ≤0.03% sodium azide.
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| Fluorescent Dye | R-PE |
| Excitation (nm) | 565 |
| Application | FCM |
Shipping and Storage
| Shipping | The product is shipped at 4°C. |
| Stability & Storage | To provide optimal stability of reagent:
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Intellectual Product Notices
Conditions: The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents.
BioSwan will not be held responsible for patent infringement or other violations that may occur with the use of our products. Purchase does not
include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this
product other than the permitted use without the express written authorization of BioSwan Company is strictly prohibited.
For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resales.
BioSwan, the BioSwan Logo and all other trademarks are property of BioSwan Laboratories, Co., Ltd.
FACS Protocol
(Optional) For Whole Blood Sample
- Pipette 5 μL Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody, PE, Ultra into the bottom of the tube.
- Add dead cell staining solution and additional fluorochrome-conjugated antibodies into the bottom of the tube.
- Pipette 100 µL of well-mixed, anticoagulated whole blood into the bottom of the tube. Mix gently and thoroughly. Note Avoid smearing sample down the side of the tube. If the sample remains on the side of the tube, it will not be stained with the reagents.
- Incubate for 25 minutes in the dark at room temperature (18-25°C).
- Pipette Red Blood Cell Lysis Solution to the tube. Mix gently and thoroughly. Incubate for 15 minutes in the dark at room temperature (18-25°C).
- Add 500 μL FACS buffer to the tube. Mix well and centrifuge at 300g for 5 minutes at room temperature (18-25°C). Aspirate supernatant
completely. - Repeat step 6 twice.
- Add a suitable amount of FACS buffer to resuspend cell and analysis by flow cytometry
(Optional) For Cell Sample
- Harvest the cells from source and wash the cells once using FACS buffer.
- Count the cells number and the viability.
- Resuspend the cell suspension to a concentration up to 1×106 nucleated cells per 100 μL of buffer.
- Add 5 μL Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody, PE, Ultra, dead cell staining solution and additional fluorochrome. Mix gently and thoroughly.
- Incubate for 25 minutes in the dark at room temperature (18-25°C).
- Add 500 μL FACS buffer to the tube. Mix well and centrifuge at 300g for 5 minutes at room temperature (18-25°C). Aspirate supernatant completely.
- Repeat step 6 twice.
- Add a suitable amount of FACS buffer to resuspend cell and analysis by flow cytometry
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Antibody solutions containing ProClin 300 should be handled with care. Do not take internally and avoid all contact with the skin, mucosa and eyes.
