$480.00

1A3 specifically binds to the scFv region of a B-cell maturation antigen (BCMA) specific mouse monoclonal antibody (mAb, clone C11D5.3). BCMA is a protein that has been reported to be selectively expressed by B-lineage cells including multiple myeloma cells1 and restrictively expressed in both normal and malignant plasma cells at high levels. The scFv region of C11D5.3 has been used to develop BCMA-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Item Information 
Catalog #  200705 
Size/Concentration  25Tests 
Price  $480.00 

Specification 
Host Species  Rabbit 
Antibody Type   Monoclonal 
Antigen Purification  Protein A 
Reactivity  Mouse 
Immunogen  scFv region of mouse mAb clone C11D5.3 
Storage Buffer  Aqueous buffered solution containing protein stabilizer and ≤0.03% sodium azide 
Fluorescent Dye  R-PE 
Excitation (nm)  565 
Application  FCM 

Shipping and Storage 

Shipping   The product is shipped at 2-8 °C.  
Stability & Storage   To provide optimal stability of reagent:   

  • Store undiluted at 2-8° C
  • Avoid prolonged exposure to light.
  • The monoclonal antibody was purified by Protein A
  • The antibody was conjugated with PE under optimum conditions, and unincorporated dye was removed.

FACS Protocol

(Optional) For Whole Blood Sample

1. Pipette 1 μL Rabbit Anti-Mouse C11D5.3 scFv Monoclonal Antibody, PE into the bottom of the tube.
2. Add dead cell staining solution and additional fluorochrome conjugated antibodies into the bottom of the tube.
3. Pipette 100 µL of well-mixed, anticoagulated whole blood into the bottom of the tube. Mix gently and thoroughly.
Note Avoid smearing sample down the side of the tube. If the sample remains on the side of the tube, it will not be stained with the reagents.
4. Incubate for 25 minutes in the dark at room temperature (18-25°C).
5. Pipette Red Blood Cell Lysis Solution to the tube. Mix gently and thoroughly. Incubate for 15 minutes in the dark at room temperature (18-25°C).
6. Add 500 μL FACS buffer to the tube. Mix well and centrifuge at 300g for 5 minutes at room temperature (18-25°C). Aspirate supernatant
completely.
7. Repeat step 6 twice.
8. Add a suitable amount of FACS buffer to resuspend cell and analysis by flow cytometry.

(Optional) For Cell Sample

1. Harvest the cells and wash the cells twice by FACS buffer.
2. Count the cells number and the viability.
3. Resuspend the cell suspension to a concentration up to 1×106 nucleated cells per 100 μL of buffer.
4. Add 1 μL Rabbit Anti-Mouse C11D5.3 scFv Monoclonal Antibody, PE, dead cell staining solution and additional fluorochrome. Mix
gently and thoroughly
5. Incubate for 25 minutes in the dark at room temperature (18-25°C).
6. Add 500 μL FACS buffer to the tube. Mix well and centrifuge at 300 g for 5 minutes at room temperature (18-25°C). Aspirate supernatant
completely.
7. Repeat step 6 twice.
8. Add a suitable amount of FACS buffer to resuspend cell and analysis by flow cytometry

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