Description

1A3 specifically binds to the scFv region of a B-cell maturation antigen (BCMA) specific mouse monoclonal antibody (mAb, clone C11D5.3). B-cell maturation antigen (BCMA) is a protein that has been reported to be selectively expressed by B-lineage cells including multiple myeloma cells and plasma cells. The scFv region of C11D5.3 has been used to develop BCMA-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Item Information
Catalog #	Size/concentration	Price
200706	100 tests	$1,750

Specification
Host species	Rabbit
Antibody type	Monoclonal
Immunogen	scFv region of mouse mAb clone C11D5.3
Reactivity	Mouse
Storage buffer	Aqueous buffered solution containing protein stabilizer and ≤0.03% sodium azide
Application	FCM

Preparation and Storage

Shipped at 2-8°C. Store at 2-8°C. Avoid freeze/thaw cycle.
The monoclonal antibody was purified by Protein A.
The antibody was conjugated with PE under optimum conditions, and unincorporated dye was removed.

Product Notices

• Since applications vary, each investigator should titrate the reagent to obtain optimal results.
• Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

FACS Protocoal

1. Harvest the cells and wash the cells once by FACS buffer (PBS containing 2% of BSA).
2. Count the cells number and the viability, aliquot up to 2×10⁵ live cells into each tube. (Note: the cell viability must be ≥ 95%.)
3. Wash the cells once by FACS buffer.
4. Resuspend cells in 100 µL of diluted Anti-Mouse C11D5.3 scFv mAb (R19M), Alexa Fluor 647 (Cat. No. 200706, 1:100 diluted in FACS buffer) for 30 min at 4°C.
5. Wash the cells 3 times by FACS buffer and resuspend the cells in 200 µL PBS per sample.
6. Transfer the cells into flow tube and analyze on Flow Cytometer. Acquisition of >10, 000 events is performed.

Technical Data Sheet