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Mouse Anti-Mouse FMC63 scFv Monoclonal Antibody (M19H), Biotin (100 tests)

Mouse Anti-Mouse FMC63 scFv Monoclonal Antibody (M19H), Biotin (100 tests)

$900.00

High affinity mouse monoclonal antibody specifically binding to the anti-CD19 scFv that was derived from the FMC63. Its robust specificity and sensitivity allows this antibody to be a perfect tool for flow cytometry and other immunostaining applications.

SKU: 300414 Category:

Description

The mouse monoclonal antibody M19H specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Item Information 
Catalog #Size/concentrationPrice
300414100 tests
$900
Specification
Host speciesMouse
Antibody typeMonoclonal
ImmunogenscFv region of a CD19-specific mouse mAb clone FMC63
ReactivityMouse
Storage bufferAqueous buffered solution containing protein stabilizer and ≤0.03% sodium azide
ApplicationFCM

Preparation and Storage

Shipped and store at 4°C. Avoid freeze/thaw cycle.
The antibody was purified by Protein A.
The antibody was conjugated with biotin under optimum conditions.

Product Notices

  • Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  • Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to
    avoid accumulation of potentially explosive deposits in plumbing.

FACS Protocoal

  1. Harvest the cells and wash the cells once by FACS buffer (PBS containing 2% of BSA).
  2. Count the cells number and the viability, aliquot up to 2×105 live cells into each tube. (Note: the cell viability must be ≥ 95%.)
  3. Wash the cells once by FACS buffer
  4. Resuspend cells in 100 µL of diluted Mouse Anti-Mouse FMC63 scFv Monoclonal Antibody, Biotin antibody (Cat. No. 300414, 1:100 diluted in FACS buffer) for 30 min at 4°C.
  5. Wash the cells once by FACS buffer
  6. Resuspend cells in 100 µL of diluted Streptavidin PE (Cat. No. SAPE-100, 1:200 diluted in FACS buffer.) for 30 min at 4°C.
  7. Wash the cells 3 times by FACS buffer and resuspend the cells in 200 µL PBS per sample.
  8. Transfer the cells into flow tube and analyze on Flow Cytometer. Acquisition of >10, 000 events is performed.

Technical Data Sheet

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