Our Science
CAR Expression Detection
Background
Evaluating CAR expression is an essential step in production of CAR-T cells. This is often done by flow cytometry by using protein L, anti-Fab antibody, target antigen or anti-idiotype antibody (anti-ID Ab) Among these common choices, anti-ID Ab is widely considered to be the best, because it offers high specificity and minimal background staining. An anti-ID Ab, mouse mab 136.20.1 (B Jena, et al., PLoS One, 2013), has been used in anti-CD19 CAR detection extensively by most top labs and in pre-clinical and clinical studies.
Cytoart’s Unique CAR-driven
Antibody Platform
Cytoart’s antibody development platform focuses on targeting the scFV region on CAR- T cells. scFV target detection is a valuable tool Cytoart implements in the development of antibodies for CAR T-cell detection reagents. Using scFV for target detection for CAR T-cell therapy offers several benefits:
HIGH SPECIFICITY:
Our ScFV-based antibodies are engineered to target the specific antigen, ensuring correct target binding and reducing off-target complications.
SENSITIVITY:
scFV target detection is highly sensitive, allowing for the detection of low levels of CAR T-cells in samples.
REAL TIME MONITORING:
ScFV target detection enables real-time monitoring of CAR T-cell activity and distribution in sample.
VERSATILITY:
ScFV target detection can be applied to various types of studies involving CAR T-cells.
how we excel
Product Advantages
Product Advantages
ACCURACY
Anti-idiotypic reagents enhance the precision of CAR-T cell targeting, ensuring more accurate identification and destruction of cancer cells.
SENSITIVITY
Anti-idiotypic reagents improve CAR-T cells’ ability to detect and eliminate cancer cells with low antigen levels, enhancing treatment effectiveness.
STABILITY
Anti-idiotypic reagents possess intrinsic stability, contributing to the reliable and prolonged shelf-life of CAR-T therapies
SPECIFICITY
These reagents increase CAR-T therapy’s precision by targeting unique cancer cell antigens, reducing off-target effects and increasing safety.
