Application of R19M

R19M: A Novel Tool with

Higher Specificity and Sensitivity

R19M(patent pending)is a high affinity rabbit monoclonalantibody specifically binding to the anti-CD19 scFv that was derived from the FMC63. Its robust specificity and sensitivity allows this antibody a perfect tool for flow cytometry and other immunostaining applications.

The surface CAR was clearly stained (red fluorescence represents the localization of anti-CD19 CAR on the cell membrane, blue fluorescence represents the nucleus) by R19M (left panel) as compared with antigen CD19/ Fc (right panel).

R19M

Strength

UNIQUE SPECIFICITY:

R19M specifically binds to FMC63 at
0.002 μg/mL, which is 1000 times more

sensitive than mAb 136.20.1.

R19M 136.20.1
Binding Started 0.002 μg/mL 2 μg/mL
Binding Saturated 0.25 μg/mL 4 μg/mL

OUTSTANDING ACCURACY:

Clear clustering of CAR-positive T cells and negative T cells is a prerequisite for accurate detection. R19M clearly defines the anti-CD19 CAR-positive cell population with high affinity, meeting drug quality control and clinical sample testing requirements.

LIMIT OF DETECTION 0.001%

Patients treated with CAR-T cells need to monitor the survival of CAR-T cells in vivo to determine the efficacy and risk of recurrence. After several months of treatment, the proportion of anti-CD19 CAR-T cells in circulation of patients is mostly below 1%. The sensitivity of detection not only affects the quality of the clinical data, but also affects the feasibility of clinical monitoring. R19M, as a powerful tool for anti-CD19 CAR-T clinical follow-up, pushed the limit of detection to 1: 100,000 (detecting 1 anti-CD19 CAR-T cell in 100,000 T cells)

Current Detection Reagents

R19M
(Bioswan Lab)		 136.20.1
(non-commercial)		 CD19/Fc
(other vendors)
Character Anti-ID Ab,
rabbit		 Anti-ID Ab,
Mouse		 Antigen
KD (nM) 0.043 NA 2.95
Limit of Deterction 0.001% 0.1% 0.5%
Stability (4 C) 12 months NA 1 month
Clinical Monitoring Long term
(>4 years)		 Long term
(>4 years)		 Short term
(<3 years)
ADA/Nab Study Yes Yes No

Related Products

Catalog Number Description Clone
200106 Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody, PE Rabbit Monoclonal
300406 Mouse Anti-Mouse FMC63 scFv Monoclonal Antibody, PE Mouse Monoclonal
500014 Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody, Biotin Rabbit Monoclonal
900010 Anti-ID Ab Services: Developing of highly sensitive and specific anti-ID Abs for CAR detection Rabbit Polyclonal/Monoclonal

our mission

For better CAR immunotherapy bioanalysis

The first commercialized anti-ID antibody (R19M) for anti-CD19 CAR T cell analysis
The only company that provide anti-ID antibodies for bioanalysis of all FDA approved CAR T cell therapies
Experienced provider of customized ID antibody development services for bioanalysis of CAR immunotherapy.

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PK / ADA / NAB

Providing comprehensive pharmacokinetic, antidrug antibody, and neutralizing antibody analyses, optimizing therapeutic windows and minimizing immunogenicity.

FCM / IHC / MSD

Our advanced Flow Cytometry (FCM), Immunohistochemistry (IHC), and Meso Scale Discovery (MSD) methodologies enable precise quantification and localization of CAR expression, enhancing treatment monitoring and effectiveness.