RE9 is a specialized rabbit monoclonal antibody with high specificity for binding to the extracellular domain of the GC33 scFv within Chimeric Antigen
Receptors (CARs). The GC33 scFv fragment is derived from a mouse monoclonal antibody that targets Glypican-3 (GPC3), a cell surface antigen implicated
in hepatocellular carcinoma (HCC). GPC3’s elevated presence in liver cancer is linked to tumor progression and metastasis, underscoring its significance
as a biomarker and therapeutic target. Ongoing clinical trials are exploring the potential of GPC3-targeted CAR-T cells in treating HCC.
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Specification |
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| Host Species | Rabbit |
| Antibody Type | Monoclonal |
| Antigen Purification | Protein A |
| Reactivity | Mouse |
| Immunogen | scFv region of a GPC3-specific mouse mAb clone GC33 |
| Storage Buffer | Aqueous buffered solution containing protein stabilizer and ≤0.05% ProClin 300.
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| Fluorescent Dye | Alexa Fluor 647 |
| Excitation (nm) | 651/667nm |
| Application | FCM |
Shipping and Storage
| Shipping | The product is shipped at 4°C. |
| Stability & Storage | To provide optimal stability of reagent:
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Intellectual Product Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on
the buyer using the purchased product solely in research conducted by the buyer and the buyer must not (1) use this product or its components
for (a) diagnostic, therapeutic or prophylactic purposes; or (b) manufacturing or quality assurance or quality control, and/or (2) sell or transfer
this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for
purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or
outlicensing@thermofisher.com.
Conditions: The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents.
BioSwan will not be held responsible for patent infringement or other violations that may occur with the use of our products. Purchase does not
include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this
product other than the permitted use without the express written authorization of BioSwan Company is strictly prohibited.
For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resales.
BioSwan, the BioSwan Logo and all other trademarks are property of BioSwan Laboratories, Co., Ltd.
FACS Protocol
(Optional) For Whole Blood Sample
1. Pipette 5 μL Anti- GPC3 CAR ldiotype Antibody, Alexa Fluor 647 into the bottom of the tube.
2. Add dead cell staining solution and additional fluorochrome conjugated antibodies into the bottom of the tube.
3. Pipette 100 µL of well-mixed, anticoagulated whole blood into the bottom of the tube. Mix gently and thoroughly.
Note Avoid smearing sample down the side of the tube. If the sample remains on the side of the tube, it will not be stained with the reagents.
4. Incubate for 25 minutes in the dark at room temperature (18-25°C).
5. Pipette Red Blood Cell Lysis Solution to the tube. Mix gently and thoroughly. Incubate for 15 minutes in the dark at room temperature (18-25°C).
6. Add 500 μL FACS buffer to the tube. Mix well and centrifuge at 300g for 5 minutes at room temperature (18-25°C). Aspirate supernatant
completely.
7. Repeat step 6 twice.
8. Add a suitable amount of FACS buffer to resuspend cell and analysis by flow cytometry.
(Optional) For Cell Sample
1. Harvest the cells and wash the cells twice by FACS buffer.
2. Count the cells number and the viability.
3. Resuspend the cell suspension to a concentration up to 1×106 nucleated cells per 100 μL of buffer.
4. Add 5 μL Anti- GPC3 CAR ldiotype Antibody, Alexa Fluor 647, dead cell staining solution and additional fluorochrome. Mix gently and thoroughly.
5. Incubate for 25 minutes in the dark at room temperature (18-25°C).
6. Add 500 μL FACS buffer to the tube. Mix well and centrifuge at 300 g for 5 minutes at room temperature (18-25°C). Aspirate supernatant
completely.
7. Repeat step 6 twice.
8. Add a suitable amount of FACS buffer to resuspend cell and analysis by flow cytometry.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Antibody solutions containing ProClin 300 should be handled with care. Do not take internally and avoid all contact with the skin, mucosa and eyes.
