Rabbit Anti-Mouse C11D5.3 scFv Monoclonal Antibody, PE (2 Sizes)

The rabbit monoclonal antibody R19M specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

Shipping and Storage 

FACS Protocol 

• Reagents Required 

• • Phosphate Buffered Saline (PBS with a pH ~7.4).  
  • FACS Buffer (PBS containing 2% of BSA).  

• • Rabbit Anti-Mouse C11D5.3 scFv Monoclonal Antibody, PE (25 tests) 

• Preparation of Reagents 

• • Rabbit Anti-Mouse C11D5.3 scFv Monoclonal Antibody, PE (25 tests) 

• • • Protein must be diluted before use for FACS. 
    • Dilute the protein at a dilution of 1:100 in FACS buffer. 

• Staining Protocol 

1. 1. Harvest the cells from source and wash the cells once using FACS buffer. 
   2. Perform a cell count and a cell viability count. Cell viability must be ≥ 95% with a cell number above 2×105 live cells. 
   3. In a round-bottom test tube, resuspend cells in 100 µL of diluted Rabbit Anti-Mouse C11D5.3 scFv Monoclonal Antibody, PE for 30 min at 4°C. 
   4. Wash the mixture of cells and antibody 3 times by centrifuge using FACS buffer. 
   5. Resuspend the stained cells in 200 µL PBS per cell sample.  
   6. Transfer the stained cell into a desired flow tube for analyzation on desired Flow Cytometer. The recommended acquisition rate is >10,000 events. 

Product Notices

• Since applications vary, each investigator should titrate the reagent to obtain optimal results.
• Caution: Antibody solutions containing ProClin 300 should be handled with care. Do not take internally and avoid all contact with the skin, mucosa
  and eyes.

Intellectual Product Notices

• Conditions: The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents. BioSwan will not be held responsible for patent infringement or other violations that may occur with the use of our products. Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of BioSwan Company is strictly prohibited. For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resales. BioSwan, the BioSwan Logo and all other trademarks are property of BioSwan Laboratories, Co., Ltd.