Ra1D6 specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
| Item Information | |
| Catalog # | 200801 |
| Size/Concentration | 25 tests |
| Price | $530.00 |
|
Specification |
|
| Antibody Type | Primary antibody, Recombinant antibody |
| Clone | Ra1D6 |
| Antigen Purification | Protein A |
| Reactivity | Mouse |
| Immunogen | scFv region of a CD19-specific mouse mAb clone FMC63 |
| Storage Buffer | Aqueous buffered solution containing protein stabilizer and ≤0.05% ProClin 300. |
| Fluorescent Dye | Alexa Fluor 647 |
| Excitation (nm) | 650 |
| Application | FCM |
Shipping and Storage
| Shipping | The product is shipped at 2-8°C. |
| Stability & Storage | To provide optimal stability of reagent:
|
Flow Cytometry FACS Protocol
- Reagents Required
- Phosphate Buffered Saline (PBS with a pH ~7.4).
- FACS Buffer (PBS containing 2% of BSA).
- Anti-Mouse FMC63 scFv Monoclonal Antibody (Ra1D6), hIgGC, Alexa Fluor 647
- Preparation of Reagents
- Anti-Mouse FMC63 scFv Monoclonal Antibody (Ra1D6), hIgGC, Alexa Fluor 647.
- The antibody must be diluted before use for FACS.
- Dilute the antibody at a dilution of 1:100 in FACS buffer.
- Anti-Mouse FMC63 scFv Monoclonal Antibody (Ra1D6), hIgGC, Alexa Fluor 647.
- Staining Protocol
- Harvest the cells from source and wash the cells once using FACS buffer.
- Perform a cell count and a cell viability count. Cell viability must be ≥ 95% with a cell number above 2×105 live cells.
- In a round-bottom test tube, resuspend cells in 100 µL of diluted Anti-Mouse FMC63 scFv Monoclonal Antibody (Ra1D6), hIgGC, Alexa Fluor 647 for 30 min at 4°C.
- Wash the mixture of cells and antibody 3 times by centrifuge using FACS buffer.
- Resuspend the stained cells in 200 µL PBS per cell sample.
- Transfer the stained cell into a desired flow tube for analyzation on desired Flow Cytometer. The recommended acquisition rate is >10,000 events.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Antibody solutions containing ProClin 300 should be handled with care. Do not take internally and avoid all contact with the skin, mucosa
and eyes.
Intellectual Product Notices
For Research Use Only. Not for use in diagnostic or therapeutic procedures
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer and the buyer must not use this product or its components:
for:- diagnostic, therapeutic or prophylactic purposes; or
- manufacturing or quality assurance or quality control.
- and/or to sell or transfer this product or its components for resale, whether or not resold for use in research.
- For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
- The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents. Bioswan will not be held responsible for patent infringement or other violations that may occur with the use of our products.
- Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product.
- Any use of this product other than the permitted use without the express written authorization of BioSwan Company is strictly prohibited.
Not for resales. BioSwan, the BioSwan Logo and all other trademarks are property of BioSwan Laboratories, Co., Ltd..
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