The rabbit polyclonal antibody BCMA specifically binds to the scFv region of a B-cell maturation antigen (BCMA) specific mouse monoclonal antibody (mAb, clone C11D5.3). B-cell maturation antigen (BCMA) is a protein that has been reported to be selectively expressed by B-lineage cells including multiple myeloma cells and plasma cells. The scFv region of C11D5.3 has been used to develop BCMA-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
| Item Information | |
| Catalog # | 500514 |
| Size/Concentration | 100 Test |
| Price | $500.00 |
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Specification |
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| Host Species | Rabbit |
| Antibody Type | Polyclonal |
| Antigen Purification | Protein A |
| Reactivity | Mouse |
| Immunogen | scFv region of a CD19-specific mouse mAb clone FMC63 |
| Storage Buffer | Aqueous buffered solution containing protein stabilizer and ≤0.05% ProClin 300 |
Shipping and Storage
| Shipping | The product is shipped at 2-8°C. |
| Stability & Storage |
To provide optimal stability of reagent:
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FACS Protocol
- Reagents Required
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- Phosphate Buffered Saline (PBS with a pH ~7.4).
- FACS Buffer (PBS containing 2% of BSA).
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- Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody, Biotin (100 Tests)
- Streptavidin PE
- Preparation of Reagents
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- Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody, Biotin (100 Tests)
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- Protein must be diluted before use for FACS.
- Dilute the protein at a dilution of 1:100 in FACS buffer.
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- Streptavidin PE
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- Dilute streptavidin PE at a dilution of 1:200 in FACS buffer.
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- Staining Protocol
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- Harvest the cells from source and wash the cells once using FACS buffer.
- Perform a cell count and a cell viability count. Cell viability must be ≥ 95% with a cell number above 2×105 live cells.
- In a round-bottom test tube, resuspend cells in 100 µL of diluted Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody, Biotin antibody for 30 min at 4°C.
- Wash the mixture of cells and antibody twice by centrifuge using FACS buffer.
- Resuspend cells in 100 µL of diluted Streptavidin PE for 30 min at 4°C.
- Resuspend the stained cells in 200 µL PBS per cell sample.
- Transfer the stained cell into a desired flow tube for analyzation on desired Flow Cytometer. The recommended acquisition rate is >10,000 events.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Antibody solutions containing ProClin 300 should be handled with care. Do not take internally and avoid all contact with the skin, mucosa
and eyes.
Intellectual Product Notices
For Research Use Only. Not for use in diagnostic or therapeutic procedures
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer and the buyer must not use this product or its components:
for:- diagnostic, therapeutic or prophylactic purposes; or
- manufacturing or quality assurance or quality control.
- and/or to sell or transfer this product or its components for resale, whether or not resold for use in research.
- For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
- The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents. Bioswan will not be held responsible for patent infringement or other violations that may occur with the use of our products.
- Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product.
- Any use of this product other than the permitted use without the express written authorization of BioSwan Company is strictly prohibited.
Not for resales. BioSwan, the BioSwan Logo and all other trademarks are property of BioSwan Laboratories, Co., Ltd..
This protocol outlines the procedure for staining cells with Cytoart’s biotinylated primary antibody and a PE-conjugated streptavidin secondary antibody for subsequent analysis by flow cytometry. Ensure all steps are performed under sterile conditions to prevent contamination. Adjustments to dilution ratios or incubation times may be required based on specific experimental conditions and antibody affinities.
FACS Analysis Protocol
Required Reagents
- Phosphate Buffered Saline (PBS, pH ~7.4).
- FACS Buffer: PBS supplemented with 2% Bovine Serum Albumin (BSA).
- Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody, Biotin-conjugated (for 100 tests).
- Streptavidin Phycoerythrin (PE) conjugate.
Reagent Preparation
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Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody, Biotin-conjugated
- Prior to use in Fluorescence-Activated Cell Sorting (FACS), this antibody must be diluted. Prepare the dilution at a ratio of 1:100 in FACS buffer.
-
Streptavidin PE Conjugate
- Dilute Streptavidin PE at a ratio of 1:200 in FACS buffer.
Staining Protocol
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Cell Preparation
- Harvest cells from the culture or specimen source, followed by a single wash using FACS buffer.
- Perform cell counting and viability assessment, ensuring a viability of ≥95% with at least 2×10^5 viable cells.
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Primary Antibody Incubation
- Resuspend the cells in 100 µL of the diluted Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody, Biotin conjugate.
- Incubate for 30 minutes at 4°C.
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Washing
- Post incubation, wash the cell-antibody mixture twice by centrifugation using FACS buffer to remove unbound antibodies.
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Secondary Antibody Incubation
- Subsequently, resuspend the cells in 100 µL of the diluted Streptavidin PE conjugate.
- Incubate for 30 minutes at 4°C.
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Final Resuspension
- After the secondary antibody incubation, resuspend the stained cells in 200 µL of PBS for each sample.
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Flow Cytometry Analysis
- Transfer the stained cells to appropriate flow cytometry tubes.
- Analyze on the flow cytometer with a recommended acquisition rate of over 10,000 events.
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