Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody, Biotin (100 Tests)

Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody, Biotin (100 Tests)

$500.00

The rabbit polyclonal antibody BCMA specifically binds to the scFv region of a B-cell maturation antigen (BCMA) specific mouse monoclonal antibody
(mAb, clone C11D5.3). B-cell maturation antigen (BCMA) is a protein that has been reported to be selectively expressed by B-lineage cells including
multiple myeloma cells and plasma cells. The scFv region of C11D5.3 has been used to develop BCMA-specific chimeric antigen receptor (CAR) T cells
utilized in clinical trials.

Description

The rabbit polyclonal antibody BCMA specifically binds to the scFv region of a B-cell maturation antigen (BCMA) specific mouse monoclonal antibody
(mAb, clone C11D5.3). B-cell maturation antigen (BCMA) is a protein that has been reported to be selectively expressed by B-lineage cells including
multiple myeloma cells and plasma cells. The scFv region of C11D5.3 has been used to develop BCMA-specific chimeric antigen receptor (CAR) T cells
utilized in clinical trials.

Item Information
Catalog # Size/concentration Price
500514  100 tests $500
Specification
Host species Rabbit
Antibody type Polyclonal
Immunogen scFv region of mouse mAb clone C11D5.3
Reactivity Mouse
Storage buffer Aqueous buffered solution containing protein stabilizer and ≤0.05% ProClin 300
Application FCM

 

Preparation and Storage

Shipped at 2-8°C. Store at 2-8°C short term (1-2 weeks). Store at -20°C in small aliquots for long term storage. Avoid freeze/thaw cycle.
The polyclonal antibody was purified by Protein A.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Antibody solutions containing ProClin 300 should be handled with care. Do not take internally and avoid all contact with the skin, mucosa
    and eyes.
  3.  Conditions: The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents.

FACS Protocol

  1. Harvest the cells and wash the cells once by FACS buffer (PBS containing 2% of BSA).
  2. Count the cells number and the viability, aliquot up to 2×105 live cells into each tube. (Note: the cell viability must be ≥ 95%.)
  3. Wash the cells once by FACS buffer.
  4. Resuspend cells in 100 µL of diluted Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody, Biotin (Cat. No. 500514, 1:100 diluted in FACS buffer)
    for 30 min at 4°C.
  5. Wash the cells once by FACS buffer.
  6. Resuspend cells in 100 µL of diluted Streptavidin PE (Cat. No. 700032, 1:200 diluted in FACS buffer) for 30 min at 4°C.
  7. Wash the cells 3 times by FACS buffer and resuspend the cells in 200 µL PBS per sample.
  8. Transfer the cells into flow tube and analyze on Flow Cytometer. Acquisition of >10, 000 events is performed.

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